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Genomics Series1 — RNA-Seq Part I

March 2 @ 1:00 pm - 4:30 pm

Part I   Monday, March 2, 2020

1. Introduction to RNA-Seq  

  1. Issues to consider
  2. Experimental design (read length, depth, replicates..)
  3. Statistics for RNASeq

2. QC and Raw read QC

  1. Daily utilities
  2. Fastq QC and Interpretation
  3. Quality trim-Trimmomatics

3. Read mapping and Read counts

  1. Aligners—tophat, bowtie, bwa, Star, HISAT2.
  2. HtSeq-count/STAR quantMode geneCounts…
  3. Post mapping QC

Lab Exercise: FastQC, Trimmomatic; HISAT2, RSeQC;

 

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Genomics Series1 — RNA-Seq Part I

Details

Date:
March 2
Time:
1:00 pm - 4:30 pm

Organizer

OARC
Phone:
9739721004
Email:
yc759@oarc.rutgers.edu
Website:
https://oarc.rutgers.edu/

Venue

Allison Road Classrooms (ARC) 116