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RNA-Seq workshop part 2 - Office of Advanced Research Computing RNA-Seq workshop part 2 - Office of Advanced Research Computing

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RNA-Seq workshop part 2

January 24, 2022 @ 1:00 pm - 4:30 pm

Computational Genomics Series by OARC

Amarel account: You need an Amarel account to participate in the lab section Apply here as soon as possible.

VPN setup: You have to be connected on Rutgers’ network or be on VPN to access Amarel resources.

To connect to the cluster: Please test and be sure it is working for you. Check the user-guide of how to get on to the cluster.

If you have questions or need help, please contact Janet Chang.

  • Data processing and filtering
    • Introduction to R and Bioconductor
    • Data processing–filtering
    • Normalization
  • Quantification and Differential expression
    • Expression Quantification
    • Statistical Models
    • Multiple test correction
  • Analysis QC (Sample QC)
    • Clustering
    • PCA
    • Heatmap
    • Volcano plot

Lab Exercise: data preparation; DE analysis with edgeR;
PCA, Clustering, heatmap, Volcano plot…

Details

Date:
January 24, 2022
Time:
1:00 pm - 4:30 pm